Dithiothreitol (DTT) and iodoacetamide (IAA) were purchased from Sigma Aldrich (St. Louis, MO). Sequencing-grade modified trypsin and chymotrypsin were purchased from Promega (Madison, WI). All other reagents were purchased from Sigma Aldrich unless indicated otherwise. Data analysis was performed using Byonic 3.5 software and manually using Xcalibur 4.2. The SARS-CoV-2 spike protein culture supernatant subunit S1 (Cat. No. 230-20407) and subunit S2 (Cat. No. 230-20408), and purified subunit S2 (Cat. No. 230-30163) were purchased from RayBiotech (Atlanta, GA).
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SARS-CoV-2 Spike Protein Analysis
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Corresponding Organization : University of Georgia
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Variable analysis
independent variables
- Dithiothreitol (DTT)
- Iodoacetamide (IAA)
- Protein identification and characterization
- All other reagents
- Positive control: SARS-CoV-2 spike protein culture supernatant subunit S1 and subunit S2
- Positive control: Purified SARS-CoV-2 spike protein subunit S2
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