Western Blotting of Cell Signaling Proteins

Western blotting was performed as previously described [17 (link)]. Primary antibodies against KIF15, FAK, FAK-397, PXN, PXN-118, SRC, SRC-418, RAB11A, PI3K-C2α, and FLAG were purchased from Abcam. The Integrin Antibody Sampler Kit and antibody against GAPDH were purchased from Cell Signaling Technology. HRP-conjugated secondary antibodies, including polyclonal goat mouse IgG and polyclonal goat rabbit IgG, were purchased from Cell Signaling. GADPH was used as a normalization loading control. Expression levels were quantified by Imaging Systems (Thermo Fisher), and the relative expression values were quantified using Image J software.