Viral RNA Quantification by RT-qPCR
Variable analysis
- Viral RNA extraction method: EZ1 apparatus running the EZ1 DSP virus kit (Qiagen)
- Viral RNA levels measured by one-step quantitative reverse transcriptase PCR assay (RT-qPCR) on the Light Cycler 480 (Roche)
- Primers, probe, and cycling conditions previously described for USUV [68] and WNV [69]
- No positive or negative controls are explicitly mentioned in the provided information.
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