Morphological Changes of Salmonella Upon Peptide Exposure

Morphological changes of bacteria following incubation with NK2A and Sc-NK2A peptides were studied by TEM. An overnight culture of Salmonella (isolate SX238) was centrifuged and resuspended in PBS. One hundred microliters of bacterial suspension were placed in a Honeycomb plate (~ 1 × 10⁷ CFU/well) and NK2A, Sc-NK2A (20 µM), and PBS were added and incubated at 37 °C with continuous shaking for 1 h. The bacterial suspensions were mixed with an equal volume of 3% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) and processed for TEM as described before^{24 (link)}. Sections were examined with a FEI Tecnai G2 Biotwin (FEI Co., Hillboro, OR) TEM and images were taken with Advanced Microscopy Technologies (AMT Inc., Danvers, MN) imaging camera.