Monkeypox virus strain Zaire (V79-I-005) was propagated and titrated as previously described [19 (link), 20 (link)]. The virus was the same lot as utilized by Mucker, et al. [21 (link)]. BS-C-1 cells (ATCC CL-26) were utilized for virus titration of blood and swabs. Inoculums were prepared in phosphate buffered saline (PBS, Gibco)(pH 7.4).
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