We employed an endotoxemic murine model of ALI induced by systemic intraperitoneal LPS as previously described [3 (link)]. Briefly, mice were fed tamoxifen 20 mg/ml in corn oil) for five consecutive days to induce endothelial cell-specific deletion of Irf1 before being left untreated for 4 weeks. Thereafter, mice were challenged with phosphate-buffered saline (PBS) vehicle control or a sublethal dose of LPS (8 mg/kg i.p., Ultrapure LPS from E. coli 0111:B4; cat. no. tlrl-3pelps, Invivogen) and then killed at various endpoints before tissue harvesting. Mice were anesthetized with pentobarbital sodium (50 mg/kg) and were decapitated.
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Murine Model of Acute Lung Injury
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Corn oil
Deletion
E coli
Endothelial cell
Endotoxemic
Irf1
Mice
Pentobarbital sodium
Phosphate
Saline
Tamoxifen
Corresponding Organization : Second Affiliated Hospital of Chongqing Medical University
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Variable analysis
independent variables
- Intraperitoneal injection of LPS (8 mg/kg)
- Tissue harvesting at various endpoints
- Mortality
- Mice were anesthetized with pentobarbital sodium (50 mg/kg) and decapitated
- Mice were fed tamoxifen (20 mg/ml in corn oil) for five consecutive days to induce endothelial cell-specific deletion of Irf1 before being left untreated for 4 weeks
- Phosphate-buffered saline (PBS) vehicle control
- Phosphate-buffered saline (PBS) vehicle control
- Phosphate-buffered saline (PBS) vehicle control
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