The human colonic adenoma cell line, Caco-2, was purchased from the American Type Culture Collection (ATCC) and cultured in RPMI-1640 (Gibco; Thermo Fisher Scientific, Inc.), with 10% fetal bovine serum (FBS, Gibco; Thermo Fisher Scientific, Inc.), 100 U/ml streptomycin/penicillin (Gibco; Thermo Fisher Scientific, Inc.) and 1% HEPES (Gibco; Thermo Fisher Scientific, Inc.) at 37°C under 5% CO2. F. nucleatum (ATCC 25586) and L. rhamnosus (ATCC 11982) were purchased from ATCC and cultured by the Wuhan Research Institute of First Light Industry (Wuhan, China). The methods of bacterial pellets and conditioned medium were as previously described (19 (link)). Caco-2 cells were treated with the supernatant of F. nucleatum and/or L. rhamnosus to examine the effects of F. nucleatum and L. rhamnosus (20 (link),21 (link)). 3-MA (5 mM) and CQ (10 µM) treatment for 2 h was used to inhibit the autophagic flux.
Protocol detail
Caco-2 Cell Responses to F. nucleatum and L. rhamnosus
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Adenoma
Autophagic
Bacterial
Caco 2 cells
Cell line
Colonic
Conditioned medium
Hepes
Human
Inhibit
Light
Pellets
Penicillin
Streptomycin
Corresponding Organization :
Other organizations : Union Hospital, Huazhong University of Science and Technology
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Variable analysis
independent variables
- Treatment of Caco-2 cells with the supernatant of F. nucleatum
- Treatment of Caco-2 cells with the supernatant of L. rhamnosus
- Treatment of Caco-2 cells with the supernatant of both F. nucleatum and L. rhamnosus
- Treatment of Caco-2 cells with 3-MA (5 mM) and CQ (10 µM) to inhibit autophagic flux
- Effects of F. nucleatum and L. rhamnosus on Caco-2 cells
- Caco-2 cells cultured in RPMI-1640 medium with 10% fetal bovine serum, 100 U/ml streptomycin/penicillin, and 1% HEPES at 37°C under 5% CO2
- Not explicitly mentioned
- Not explicitly mentioned
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