Western Blot Analysis of Signaling Pathways

For Western blotting analysis, protein extraction was performed as previously described (52 (link)). Antibodies against P-JAK2 (Tyr^1007/1008) (#3776), P-STAT3 (Tyr⁷⁰⁵) (#9131), STAT3 (#4904), P-AKT (Ser⁴⁷³) (#9271), AKT (#4691), P-ERK1/2 (Thr²⁰²/Tyr²⁰⁴) (#9101), ERK1/2 (#4695), DNMT1 (#5032), c-MYC (#5605), PIM1 (#3247), BCL2 (#4223), MCL-1 (#5453), and β-actin (#4970) were purchased from Cell Signaling Technology. Antibodies against JAK2 (sc-294), P-STAT5 (Tyr⁶⁹⁴) (sc11761), and STAT5 (sc-835) were purchased from Santa Cruz Biotechnology, and antibody against CCRL2 (LS-C382506) was purchased LSBio.

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Karantanos T., Teodorescu P., Perkins B., Christodoulou I., Esteb C., Varadhan R., Helmenstine E., Rajkhowa T., Paun B.C., Bonifant C., Dalton W.B., Gondek L.P., Moliterno A.R., Levis M.J., Ghiaur G, & Jones R.J. (2022). The role of the atypical chemokine receptor CCRL2 in myelodysplastic syndrome and secondary acute myeloid leukemia. Science Advances, 8(7), eabl8952.

Publication 2022

P-JAK2 (Tyr1007/1008) P-STAT3 (Tyr705) STAT3 P-AKT (Ser473) P-ERK1/2 (Thr202/Tyr204) ERK1/2 DNMT1 c-MYC BCL2 MCL-1 β-actin STAT5 (sc-835)

Actin Antibodies Antibody Bcl2 C myc Ccrl2 Dnmt1 Erk1 Jak2 Pim1 Protein Stat3 Stat5 Western blotting analysis

Corresponding Organization :

Other organizations : Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University, Johns Hopkins Hospital

Top 5 similar protocols

Variable analysis

independent variables

Protein extraction

dependent variables

P-JAK2 (Tyr1007/1008)
P-STAT3 (Tyr705)
STAT3
P-AKT (Ser473)
P-ERK1/2 (Thr202/Tyr204)
ERK1/2
DNMT1
C-MYC
MCL-1
CCRL2

control variables

β-actin

Annotations

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