ChIP-qPCR Analysis of H3K4me3

Native ChIP for H3K4^me3 (Abcam, ab1012) was performed in MDA-MB-231 cells that were either untreated, treated with 1 μM or 2.5 μM Tat-SID, or transfected with Scr-shRNA or PF1-shRNA as previously described [65 (link)]. Input DNA was used as control for the background. DNA obtained from input or immunoprecipitated DNA was analyzed by real-time PCR using primers mapping to the CD44, ITGA6 and SNAI2 promoter regions. Percent Input method was used for analysis. The following PCR primers were used: CD44-F 5′-TCACATAGCCAGGAGCAGTG-3′; CD44-R 5′-AAATCCTTCCCTCCCTGAAA-3′; ITGA6-F 5′-ACCTCCCAGGAGAAAGAGGA-3′; ITGA6-R 5′-GCGACTAAGCGCCAAAATAC-3′, SNAI2-F 5′-CAGACCCTGGTTGCTTCAA-3′; SNAI2-R 5′-CTTCATGCAAATCCAACAGC-3′; RPL30-F 5′-GCAAAGCGAAATTGGTCATT-3′; RPL30-R 5′-CTGTTTTCACTCCTGCCACA-3′.