Total RNA isolation and RT‐qPCR assays were performed as described previously (Zhang et al., 2022 (link)). Briefly, total RNA was extracted from aphid tissues, tobacco or wheat leaves using TRIzol Reagent (Thermo Fisher, USA) according to the manufacturer's protocols. First‐strand cDNA synthesis was conducted using the HiScript III First‐Strand cDNA Synthesis Kit (+gDNA wiper) (Vazyme, China), and RT‐qPCR was performed on the ABI Prism 7500 Real‐Time PCR System using SYBR Premix Ex Taq Kit (TaKaRa, Japan) and sequence‐specific primers (Table S2 ). Three replicates were conducted for each treatment, and each replicate contains three technical replicates.
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