Cryo-EM Imaging of Carboxysome
Corresponding Organization :
Other organizations : University of Basel
Variable analysis
- Dilution of Full-length
Cth ACC to 0.01 mg ml−1
- Imaging of
Cth ACC on a CM-200 microscope (Philips) equipped with a TVIPS F416 4k CMOS camera (Tietz Video and Image Processing Systems) - Initial image processing and particle picking using Xmipp
- Alignment and classification of 22,257 particles into 48 2D class averages using the maximum-likelihood target function in Fourier space (MLF2D)
- Re-alignment and classification of 18,031 particles into 36 classes using MLF2D with a high-resolution cutoff of 30 Å
- 30 mM MOPS pH 7.0
- 200 mM ammonium sulfate
- 5% glycerol
- 10 mM dithiothreitol
- Staining with 2% uranyl acetate
- Voltage of 200 kV
- Magnification of × 50,000 resulting in a pixel size of 2.14 Å
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