Example 2
Lyophilization process: Measure amount of desired exosome solution. Add 1%-10% (e.g., 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%) by volume of a stabilizing agent selected from sucrose, mannitol or trehalose.
Carnosine functions as a biological pH buffer and antioxidant. Carnosine has protein stability benefits and prevents aggregation along with vasodilation benefits. Magnesium citrate is a magnesium salt used to regulate pH along with preservation.
Manufacturing Process for 1000 capsules each at 100 mg containing 5 mg exosome, 20 mg L-carnosine, and 75 mg magnesium citrate.
Materials: exosomes 5 grams, L-carnosine 20 grams, magnesium citrate 75 grams.
Steps for manufacturing: (1) calibrate scale; (2) accurately weigh out each of the materials using lab scoop; (3) sieve each material into one weighing dish; (4) add material to V-Blender; (5) turn on V-Blender and blend for 60 minutes; (6) empty material from V-Blender into clean weighing dish; (7) fill capsules with blended material.
