Metabolic Profile Analysis of Diets

The chemical composition of diets and whole body composition were measured as previously described by Song et al. [12 ]. Plasma glucose, triglycerides, lactate and free fatty acids were analysed with Glucose RTU (BioMerieux), PAP 150 (Biomerieux), Lactate PAP (Biomerieux) and NEFA C (Fuji Chemicals GmbH) kits, respectively, according to the recommendations of each manufacturer (n = 9 samples per experimental treatment). Total plasma free amino acid concentrations were determined by the ninhydrin reaction according to the method of Moore with glycine as standard [27 (link)]. Muscle hexokinase (HK) and phosphofructokinase (PFK) activities were measured according to Borges et al. [28 (link)] (n = 9 samples per experimental treatment). Muscle pyruvate kinase (PK) activity was determined as previously described by Panserat et al. [29 ] (n = 9 samples per experimental treatment). Liver and muscle glycogen levels were measured by a hydrolysis technique as reported by Good et al. [30 ] (n = 9 samples per experimental treatment). Liver and muscle glycogen was determined by a hydrolysis technique previously described by Good et al. [30 ] and Song et al. [12 ] (n = 6 samples per experimental treatment).