Comprehensive Protein Analysis of Cellular Responses

Whole-cell extracts of untreated and treated cells were used in Western blot analysis as described previously [53 (link)]. Antibodies against p-EGFR (cat no. 3777; 1 : 1000), EGFR (cat no. 2232; 1 : 1000), p-MET (cat no. 3077; 1 : 1000), MET (cat no. 8198; 1 : 1000), p-HER3 (cat no. 4791; 1 : 1000), HER3 (cat no. 12708; 1 : 1000), p-AKT (cat no. 9271; 1 : 1000), AKT (cat no. 9272; 1 : 1000), GAPDH (cat no. 5174; 1 : 1000), vimentin (cat no. 5741; 1 : 1000), snail (cat no. 3879; 1 : 1000), and MMP-9 (cat no. 13667; 1 : 1000) were from Cell Signaling Technology (Danvers, Massachusetts, USA). P-ERK1/2 (cat no. sc7383; 1 : 1000) and ERK2 (cat no. sc1647; 1 : 1000) antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas, USA).