Mito-QC Mitophagy Visualization Protocol

Mito-QC mice express a mCherry-GFP tandem protein targeted to the mitochondrial outer membrane. When mitochondria are degraded in the lysosomes, the acidic environment quenches the GFP but not the mCherry fluorescence and mitophagy can be assessed on the basis of unbiased mCherry/GFP ratio or by selected gating strategy (23, 24 (link)). When stated cells were treated with FOXO inhibitor AS1842856 (50 nmol/L; S8222, Selleckchem) or mitophagy inhibitor mDivi (10 µmol/L; HY-15886, MedChem Express).

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Ginefra P., Hope H.C., Chiang Y.H., Nutten S., Blum S., Coukos G, & Vannini N. (2024). Urolithin-A Promotes CD8+ T Cell–mediated Cancer Immunosurveillance via FOXO1 Activation. Cancer Research Communications, 4(5), 1189-1198.

Publication 2024

AS1842856 S8222 HY-15886

Corresponding Organization :

Other organizations : University of Lausanne, Ludwig Cancer Research, Frontier Science Foundation

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Variable analysis

independent variables

Treatment with FOXO inhibitor AS1842856 (50 nmol/L)
Treatment with mitophagy inhibitor mDivi (10 µmol/L)

dependent variables

Mitophagy assessed on the basis of unbiased mCherry/GFP ratio
Mitophagy assessed by selected gating strategy

control variables

Mito-QC mice express a mCherry-GFP tandem protein targeted to the mitochondrial outer membrane

controls

Positive control: Untreated Mito-QC mice
Negative control: Not specified

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