Culturing Murine Brain Endothelial Cells

Murine microvascular brain endothelial cells (cerebEND) [23 (link),24 (link),25 (link),26 (link)] were grown in Dulbecco’s Modified Eagle’s Medium (DMEM) supplied with 1% penicillin/streptomycin and 10% fetal calf serum (FCS) on gelatin-coated 6- or 96-well plates (Thermo Fisher Scientific, Waltham, MA, USA). They were cultured in a 37 °C incubator (Heracell VIOS 250i, Thermo Fisher Scientific, USA) until 95% confluent as observed under the microscope.

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Reschke M., Salvador E., Schlegel N., Burek M., Karnati S., Wunder C, & Förster C.Y. (2022). Isosteviol Sodium (STVNA) Reduces Pro-Inflammatory Cytokine IL-6 and GM-CSF in an In Vitro Murine Stroke Model of the Blood–Brain Barrier (BBB). Pharmaceutics, 14(9), 1753.

Publication 2022

Heracell VIOS 250i

Brain Eagle Endothelial cells Fetal calf serum Gelatin Microscope Murine Penicillin Streptomycin

Corresponding Organization : Robert Bosch Hospital

Other organizations : University of Würzburg

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cell growth and confluence

control variables

Growth medium composition (Dulbecco's Modified Eagle's Medium (DMEM) with 1% penicillin/streptomycin and 10% fetal calf serum (FCS))
Substrate (gelatin-coated 6- or 96-well plates)
Incubation conditions (37 °C incubator)

controls

No positive or negative controls were explicitly mentioned in the provided information.

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