Expansion and Maintenance of NK Cell Lines

Human NK cell lines, NK-92 (ATCC #CRL-2407) and PTA-6967 (ATCC #PTA-6967) were maintained in NK cell media as described previously (12 (link)). NK cell media [Alpha Minimum Essential medium without ribonucleosides and deoxyribonucleosides but with 2 mM L-glutamine and 1.5 g/L sodium bicarbonate (Gibco #12561-056) was supplemented with 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 12.5% horse serum (Gibco #16050-122), 12.5% fetal calf serum (Gibco #10438-034), and 100 U/mL recombinant IL-2 (R&D Systems #202-IL)].

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Lagassé H.A., Ou J., Sauna Z.E, & Golding B. (2024). Factor VIII moiety of recombinant Factor VIII Fc fusion protein impacts Fc effector function and CD16+ NK cell activation. Frontiers in Immunology, 15, 1341013.

Publication 2024

PTA-6967

Corresponding Organization :

Other organizations : Center for Biologics Evaluation and Research, United States Food and Drug Administration

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Variable analysis

independent variables

NK cell lines (NK-92 and PTA-6967)

dependent variables

Not explicitly mentioned

control variables

NK cell media composition (Alpha Minimum Essential medium with 2 mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.2 mM inositol, 0.1 mM 2-mercaptoethanol, 0.02 mM folic acid, 12.5% horse serum, 12.5% fetal calf serum, and 100 U/mL recombinant IL-2)

controls

No positive or negative controls were explicitly mentioned in the input protocol.

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