Immunoprecipitation Analysis of Chromatin Regulators

Immunoprecipitations were carried out as described (46 (link)) using anti-MSX2 (HPA005652, Sigma-Aldrich) anti-ARID1A (12354, Cell Signaling), anti-SMARCA4 (ab110641, Abcam), anti-trimethyl histone H3 (Lys27) (07-442, Merck Millipore), anti-trimethyl histone H3 (Lys9) (07-449, Merck Millipore), and anti-histone H3K27 (ACM-39685, Active Motif/THP) antibody; for details, including the bioinformatic analysis, see SI Appendix.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Hornbachner R., Lackner A., Papuchova H., Haider S., Knöfler M., Mechtler K, & Latos P.A. (2021). MSX2 safeguards syncytiotrophoblast fate of human trophoblast stem cells. Proceedings of the National Academy of Sciences of the United States of America, 118(37), e2105130118.

Publication 2021

HPA005652 anti-ARID1A anti-trimethyl histone H3 (Lys27) anti-trimethyl histone H3 (Lys9)

Antibody Arid1a Histone Histone h3 Immunoprecipitations Smarca4

Corresponding Organization : Medical University of Vienna

Other organizations : Research Institute of Molecular Pathology

Top 5 similar protocols

Variable analysis

independent variables

Anti-MSX2 (HPA005652, Sigma-Aldrich)
Anti-ARID1A (12354, Cell Signaling)
Anti-SMARCA4 (ab110641, Abcam)
Anti-trimethyl histone H3 (Lys27) (07-442, Merck Millipore)
Anti-trimethyl histone H3 (Lys9) (07-449, Merck Millipore)
Anti-histone H3K27 (ACM-39685, Active Motif/THP)

dependent variables

Immunoprecipitations

control variables

Immunoprecipitations were carried out as described (46 (link))

controls

Positive controls: None specified.
Negative controls: None specified.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!