Genomic DNA was extracted from whole blood using the Qiagen Flexigene DNA Kit (Qiagen). The rs12979860 SNP was genotyped by allelic discrimination using a TaqMan Custom SNP Genotyping Assay (Applied Biosystems) on an ABI 7500 Real-Time PCR System (Applied Biosystems). rs368234815 was genotyped by Sanger sequencing using BigDye Terminator v3.1 (Applied Biosystems) according to the manufacturer’s protocol, or by allelic discrimination using a TaqMan Custom SNP Genotyping Assay (Applied Biosystems) as previously described.15 (link)
Protocol detail
Genotyping of rs12979860 and rs368234815
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Corresponding Organization :
Other organizations : National Institutes of Health, National Institute of Diabetes and Digestive and Kidney Diseases
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Variable analysis
independent variables
- Rs12979860 SNP
- Rs368234815
- Genotype
- Genomic DNA extracted from whole blood using the Qiagen Flexigene DNA Kit
- TaqMan Custom SNP Genotyping Assay (Applied Biosystems) used for allelic discrimination
- Sanger sequencing using BigDye Terminator v3.1 (Applied Biosystems) used for genotyping
- Positive control: Not specified
- Negative control: Not specified
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