Assessing Neuronal Cell Phenotypes

To assess terminal cell phenotypes, maximum intensity projection GFP images and the corresponding minimum intensity projection brightfield images of dorsal branch terminal cells were manually assessed and scored for dilation in the transition zone or other phenotypes in the terminal cells (refer to Fig. 1a, and Supplementary Tables 2 and 3 for results). Dilation rate was assessed as the percentage of terminal cell transition zones that had at least one dilation. If multiple dilations were observed in a terminal cell transition zone, it was not scored any differently to a terminal cell transition zone with a single dilation. Each data point graphed represents the mean dilation rate of the indicated genotypes.