Characterization of GelMA Hydrogels

The GelMA was dissolved in D₂O for analysis using 400-Hz nuclear magnetic resonance (Bruker AVANCE AV Ⅱ-400 MHz). The degree of methacrylate substitution was determined by the formula: 1 − (lysine integration signal of GelMA/lysine integration signal of unsubstituted Gelatin) × 100% (Brinkman et al., 2003 (link); Nichol et al., 2010 (link); Loessner et al., 2016 (link)). The morphology of the GelMA hydrogel was observed by scanning electron microscope (SEM). Dynamic Mechanical Analyzer (TA Instruments, Q-800, USA) was used to test the storage modulus and loss modulus of the GelMA hydrogel. The rheological properties of the GelMA hydrogel were analyzed by rheometer (MCR302, Anton Paar) (Zhou, 2021 (link)).

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Li J., Wang W., Li M., Song P., Lei H., Gui X., Zhou C, & Liu L. (2021). Biomimetic Methacrylated Gelatin Hydrogel Loaded With Bone Marrow Mesenchymal Stem Cells for Bone Tissue Regeneration. Frontiers in Bioengineering and Biotechnology, 9, 770049.

Publication 2021

AVANCE AV Dynamic Mechanical Analyzer MCR302

Gelatin Hydrogel Lysine Methacrylate Nuclear magnetic resonance Scanning electron microscope

Corresponding Organization : West China Hospital of Sichuan University

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Variable analysis

independent variables

Gelatin methacrylate (GelMA) dissolved in D2O

dependent variables

Degree of methacrylate substitution
Morphology of the GelMA hydrogel
Storage modulus of the GelMA hydrogel
Loss modulus of the GelMA hydrogel
Rheological properties of the GelMA hydrogel

control variables

Unsubstituted gelatin (used as a reference for degree of methacrylate substitution)

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