Lymphocyte Antigen Profiling by Flow Cytometry

Cell surface expression of lymphocyte antigens was performed by monoclonal antibody staining of freshly isolated MMCs and PBMCs, followed by flow cytometry using a BDLSRII instrument (Becton-Dickinson, Palo Alto, California, United States) with analysis using FlowJo software (TreeStar). Monoclonal antibodies used in this study included: anti-human CD3-Pacific Blue (PB) (clone UCHT1) (BD Pharmingen), anti-human CD4-Alexa700 (clone RPA T4) ((BD Pharmingen), anti-human CD8 APC-Cy7 (clone SK1) (BD Pharmingen), anti-human CD38-PE (clone HIT2) (BD Pharmingen), and anti-human Ki67-PE (clone B56) (BD Pharmingen), and the appropriate isotype controls. Flow cytometric analyses were performed as previously described [3 (link)].

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Mehandru S., Deren S., Kang S.Y., Banfield A., Garg A., Garmon D., LaMar M., Evering T.H, & Markowitz M. (2015). Behavioural, Mucosal and Systemic Immune Parameters in HIV-infected and Uninfected Injection Drug Users. Journal of addiction research & therapy, 6(4), 1-8.

Publication 2015

BDLSRII anti-human CD3-Pacific Blue (PB) (clone UCHT1) anti-human CD4-Alexa700 (clone RPA T4) anti-human CD8 APC-Cy7 (clone SK1) anti-human CD38-PE (clone HIT2) anti-human Ki67-PE (clone B56)

Anti cd3 Cell surface antigens Clone Flow cytometric Human Isotype Lymphocyte Mmcs Monoclonal antibodies

Corresponding Organization :

Other organizations : Icahn School of Medicine at Mount Sinai, New York College of Health Professions, New York University, Aaron Diamond AIDS Research Center, Rockefeller University

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Variable analysis

independent variables

Monoclonal antibodies used for cell surface expression of lymphocyte antigens

dependent variables

Cell surface expression of lymphocyte antigens (CD3, CD4, CD8, CD38, Ki67)

control variables

Freshly isolated MMCs and PBMCs

controls

Appropriate isotype controls were used for the monoclonal antibodies

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