Pharmacological Modulation of Immune Responses

Salmeterol (cat. MB1325, Meilune, Dalian, China) was stored at −20°C and dissolved in PBS before oral gavage administration (10 mg/kg).⁴⁹ (link) α-Methyl-L-tyrosine (α-MLT; cat. M107878, Aladdin, Shanghai, China) was formulated into a 3-mg/mL suspension in sterile PBS and then administered by means of oral gavage (300 mg/kg/d, 2.5-mL solution).⁵⁰ (link) Sterile PBS was used as the vehicle. For Ly6C^hi monocyte depletion, mice were IP injected with 2 mg/kg of a CCR2 antagonist (cat. 227016, Millipore, Burlington, MA, USA).⁵¹ (link) Mice pretreated with CCR2 antagonist for 24 hours were used to create a corneal abrasion model. For blocking β2-adrenergic receptors, butaxamine (cat. B1385, Sigma-Aldrich) was administered via intraperitoneal injection at a dose of 2.5 mg/kg.⁵² (link)