For fluorescence microscopy, yeast cells were grown in SD+CA medium containing appropriate supplements unless otherwise indicated. Cells were analyzed using two different fluorescence microscopy systems, as described previously (Mochida et al., 2020 (link)). The images in Figs. 1 D, S1 A, S5 B, and S6 were acquired using an inverted microscope (IX81; Olympus) equipped with an electron-multiplying charge-coupled device camera (ImagEM C9100-13; Hamamatsu Photonics), a 150× objective lens (UAPON 150XOTIRF, NA/1.45; Olympus), a Z drift compensator (IX3-ZDC2; Olympus), appropriate lasers and filters, and MetaMorph software (Molecular Devices). For time-lapse imaging, cells were grown in the glass-bottom dish and kept at 30°C using a stage-top incubator (TOKAI HIT). The images in Fig. S5 B were deconvoluted by AutoQuant X3 software (Media Cybernetics). All other fluorescence microscopy images were acquired using a Delta Vision Elite microscope system (GE Healthcare) equipped with a scientific complementary metal-oxide-semiconductor camera (pco.edge 5.5; PCO AG), a 60× objective lens (PLAPON, NA/1.42; Olympus), a 100× objective lens (UPlanSApo, NA/1.40; Olympus), and SoftWoRx software. Images acquired by a Delta Vision were deconvoluted using SoftWoRx software. All acquired images were analyzed using Fiji.