HD11 cells were obtained from the laboratory of Dr. Mark Parcells (University of Delaware). The cells were maintained at 37 °C, 5% CO2, and 95% humidity in Iscove’s Modified Dulbecco’s Media (IMDM; GE Life Sciences, Logan, UT, USA) with 10% fetal bovine serum (Midsci, Valley Park, MO, USA) and 1% 1.5 mM L-glutamine containing penicillin and streptomycin (Gibco, Grand Island, NY, USA).
Salmonella Enteritidis, resistant to nalidixic acid and novobiocin, was donated by Dr. Haiqi He, US Department of Agriculture Research Service. The strain was conserved at −80 °C in tryptic soy broth (TSB; Becton, Dickinson and Company, Sparks, MD, USA) and supplemented with 20% (v/v) glycerol. The day prior to the experiments, it was thawed and cultured overnight in TSB supplemented with 25 μg/mL novobiocin and 20 μg/mL nalidixic acid (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C.
Salmonella Enteritidis, resistant to nalidixic acid and novobiocin, was donated by Dr. Haiqi He, US Department of Agriculture Research Service. The strain was conserved at −80 °C in tryptic soy broth (TSB; Becton, Dickinson and Company, Sparks, MD, USA) and supplemented with 20% (v/v) glycerol. The day prior to the experiments, it was thawed and cultured overnight in TSB supplemented with 25 μg/mL novobiocin and 20 μg/mL nalidixic acid (Sigma-Aldrich, St. Louis, MO, USA) at 37 °C.
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