Reconstitution of Lipid Membranes with Glycolipids

C8 lactosyl(β)-ceramide (LacCer), 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and cholesterol were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Atto 647N 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) was purchased from Sigma-Aldrich (Darmstadt, Germany). Globotriaosylceramide (Gb3) was obtained from Matreya (State College, PA, USA). Shiga toxin B-subunit (StxB) was kindly provided by enGenes Biotech (Vienna, Austria). UDP-Glo Glycosyltransferase assay kit and ultra-pure UDP-Galactose (UDP-Gal) were from Promega (Charbonnières-les-Bain, France). UDP-Gal for QCM-D analysis was purchased from Carbosynth (Compton, UK). HisTrap FF column was purchased from Cytiva (Marlborough, MA, USA) and Superdex 200 Increase 10/300 GL from GE Healthcare (Chicago, MA, USA). All other chemical reagents were of analytical or liquid chromatography grade.

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Jabeguero D., Siukstaite L., Wang C., Mitrovic A., Pérez S., Makshakova O., Richter R.P., Römer W, & Breton C. (2023). Enzymatic Glyco-Modification of Synthetic Membrane Systems. Biomolecules, 13(2), 335.

Publication 2023

cholesterol Atto 647N 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) Globotriaosylceramide (Gb3) UDP-Glo Glycosyltransferase assay kit ultra-pure UDP-Galactose (UDP-Gal) HisTrap FF column Superdex 200 Increase 10/300 GL

Alabaster Assay Ceramide Cholesterol Dioleoyl phosphoethanolamine Dopc Globotriaosylceramide Glycosyltransferase Lipids Liquid chromatography Promega Shiga toxin Sn glycero 3 phosphocholine Subunit Udp d galactose

Corresponding Organization :

Other organizations : Centre de Recherches sur les Macromolécules Végétales, University of Freiburg, University of Leeds, Russian Academy of Sciences

Top 5 similar protocols

Variable analysis

independent variables

C8 lactosyl(β)-ceramide (LacCer)
1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)
Cholesterol
Atto 647N 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE)
Globotriaosylceramide (Gb3)
Shiga toxin B-subunit (StxB)
UDP-Gal

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

None specified

negative controls

None specified

Annotations

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