SKOV3ip1 and HeyA8 cells were provided by Dr. Gordon Mills (M.D. Anderson Cancer Center, Houston, TX). HeyA8-MDR was a gift from Dr. Anil Sood (M.D. Anderson Cancer Center, Houston, TX) (30 (link)). SKOV3-Taxol resistant cells (TR) were provided by Dr. Zhenfeng Duan (MGH, Boston MA) (31 (link)). Tyk-nu cells were provided by Dr. Kenjiro Sawada (Osaka University, Japan) (32 (link)). The Tyk-nu carboplatin-resistant (R) cell line (33 (link)) was purchased from JCRB cell bank. Tyk-nu and Tyn-nu-R cells were cultured in Eagle’s Minimum Essential Medium with 15% fetal bovine serum, 1% penicillin–streptomycin and 1% L-glutamine (34 (link)). All other human OvCa cell lines were maintained in Dulbecco’s modified Eagle’s medium with 10% FBS and 1% penicillin-streptomycin solution at 37°C with 5% CO2. All cell lines were authenticated using the commercial service, CellCheck (IDEXX Bioresearch). Samples were confirmed to be of human origin and no mammalian inter-species contamination was detected. The alleles for 9 short tandem repeat (STR) markers were determined and the results were compared to the profiles from DSMZ, ATCC, JCRB, and RIKEN STR databases.
Protocol detail
Characterization of ovarian cancer cell lines
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Alleles
Cancer
Carboplatin
Cell lines
Cells
Eagle
Glutamine
Human
Mammalian
Penicillin
Short tandem repeat
Streptomycin
Taxol
Corresponding Organization : University of Chicago
Other organizations : Northeastern University
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Variable analysis
independent variables
- SKOV3ip1 cells
- HeyA8 cells
- HeyA8-MDR cells
- SKOV3-Taxol resistant cells (TR)
- Tyk-nu cells
- Tyk-nu carboplatin-resistant (R) cell line
- Not explicitly mentioned
- Cell culture conditions: Eagle's Minimum Essential Medium with 15% fetal bovine serum, 1% penicillin–streptomycin and 1% L-glutamine for Tyk-nu and Tyn-nu-R cells
- Cell culture conditions: Dulbecco's modified Eagle's medium with 10% FBS and 1% penicillin-streptomycin solution at 37°C with 5% CO2 for all other human OvCa cell lines
- Cell line authentication: Samples were confirmed to be of human origin and no mammalian inter-species contamination was detected, and the alleles for 9 short tandem repeat (STR) markers were determined and compared to profiles from DSMZ, ATCC, JCRB, and RIKEN STR databases
- Not explicitly mentioned
- Not explicitly mentioned
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