Sauchinone Purity Characterization and Evaluation

Sauchinone (over 98% purity) was provided by Professor Chin Y.-W. (Pharmacognosy Laboratory of Dongguk University, Seoul, Korea) according to the modification of previously reported method [48 (link)]. Hydroxybupropion, 4-hydroxymephenytoin, 6-hydroxychlorzoxazone, S-mephenytoin, and pooled human liver microsomes from a mixed pool of 50 donors (HLMs; BD Ultra Pool HLM 50, cat. 452156) were purchased from BD Gentest Co. (Woburn, MA, USA). Acetaminophen, chlorzoxazone, clopidogrel, coumarin, furafylline, 6α-hydroxytestosterone, 7-hydroxycoumarin, ketoconazole, 4-methylpyrazole, omeprazole, paroxetine, phenacetin, quinidine, sibutramine, sulfaphenazole, testosterone, tetraethylthiuram disulfide, ticlopidine, tienilic acid, tolbutamide, tranylcypromine, verapamil, and reduced form of NADPH (as a tetrasodium salt) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dextrorphan and 4-hydroxytolbutamide were purchased from Toronto Research Chemicals Inc. (North York, ON, Canada). Bupropion, carbamazepine (internal standard, IS), dextromethorphan, fluvoxamine, fluoxetine, and 8-methoxypsoralen were purchased Wako Co. (Tokyo, Japan). All other chemicals and reagents used were of analytical grade.