Piezo2 Genetic Manipulation Protocols

Piezo2^GFP-IRES-Cre (Piezo2^GFP)^{17 (link)}; PvalbCre (The Jackson Laboratory, stock# 8069); Piezo2^fl/fl17; HoxB8Cre^{23 (link)}; Ai9 (The Jackson Laboratory, stock# 7909).
Note that PvalbCre;Piezo2^cKO and HoxB8Cre;Piezo2^cKO lines refer to
PvalbCre;Piezo2^fl/− and HoxB8Cre;Piezo2^fl/− mice. For these Piezo2 cKO lines, Piezo2^fl/+ mice were used as wild type controls. For Figs. 2c and 3 & Supplementary Figs. 4e and 6, PvalbCre;Ai9;Piezo2^cKO and PvalbCre;Ai9 (WT control) mice were used to label proprioceptive neurons.

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Woo S.H., Lukacs V., de Nooij J.C., Zaytseva D., Criddle C.R., Francisco A., Jessell T.M., Wilkinson K.A, & Patapoutian A. (2015). Piezo2 is the principal mechanotransduction channel for proprioception. Nature neuroscience, 18(12), 1756-1762.

Publication 2015

PvalbCre HoxB8Cre

Figs Ires Mice Neurons Proprioceptive

Corresponding Organization :

Other organizations : Scripps Research Institute, Howard Hughes Medical Institute, Columbia University, San Jose State University

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Variable analysis

independent variables

Piezo2^GFP-IRES-Cre (Piezo2^GFP)
PvalbCre
Piezo2^fl/fl
HoxB8Cre

dependent variables

Not explicitly mentioned

control variables

Piezo2^fl/+ mice were used as wild type controls for PvalbCre;Piezo2^cKO and HoxB8Cre;Piezo2^cKO lines
PvalbCre;Ai9 (WT control) mice were used to label proprioceptive neurons

controls

Positive control: PvalbCre;Ai9 (WT control) mice were used to label proprioceptive neurons
Negative control: Piezo2^fl/+ mice were used as wild type controls for PvalbCre;Piezo2^cKO and HoxB8Cre;Piezo2^cKO lines

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