Protocol detail

Genetic tools for Drosophila Wnt signaling

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UAS-GFP-wg[27] (link), UAS-DFz2 and UAS-DFz2N[28] (link), UAS-Dlp-HA[16] (link), wg{KO; Gal4}[29] (link), wg{KO; wg-HA}[30] (link), wg^cx4, sfl⁰³⁸⁴⁴, Df(3R)Exel6193, Df(3R)BSC527, Df(3R)BSC619, Mi{ET1}CG13830^MB00767, neur-lacZ (neur^A101), UAS-Shi^ts, UAS-lacZ, UAS-myrRFP, UAS-FLP, tub-p-Gal4, ap-Gal4, en-Gal4, hh-Gal4, ptc-Gal4, MS1096-Gal4, and nub-Gal4 were obtained from the Bloomington Drosophila Stock Center. The cow^5Δ allele was generated by imprecise excision of the Minos transposable element Mi{ET1}CG13830^MB00767 from the 3′-UTR of cow (see Figure S1A).

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Chang Y.H, & Sun Y.H. (2014). Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless. PLoS ONE, 9(10), e111573.

Publication 2014

UAS-GFP UAS-Shits UAS-lacZ UAS-myrRFP UAS-FLP tub-p-Gal4 ap-Gal4 en-Gal4 hh-Gal4 ptc-Gal4 MS1096-Gal4 nub-Gal4

Allele Drosophila Lacz Transposable element

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Protocol cited in 1 other protocol

Variable analysis

independent variables

UAS-GFP-wg
UAS-DFz2
UAS-DFz2N
UAS-Dlp-HA
Wg{KO; Gal4}
Wg{KO; wg-HA}
Wg^cx4
Sfl^03844
Df(3R)Exel6193
Df(3R)BSC527
Df(3R)BSC619
Mi{ET1}CG13830^MB00767
Cow^5Δ
UAS-Shi^ts
UAS-lacZ
UAS-myrRFP
UAS-FLP
Tub-p-Gal4
Ap-Gal4
En-Gal4
Hh-Gal4
Ptc-Gal4
MS1096-Gal4
Nub-Gal4

dependent variables

Not explicitly mentioned

control variables

Neur-lacZ (neur^A101)
UAS-Shi^ts
UAS-lacZ
UAS-myrRFP
UAS-FLP

Annotations

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