WGA-labeled GCX in aorta sections were visualized using a Zeiss LSM 710 Confocal Laser Scanning microscope at 63X/oil magnification. GCX coverage and thickness were quantified as previously described [17 (link)]. ZO-1, PECAM-1, E-selection, and eNOS labeled en face aorta tissue samples were also visualized by confocal microscopy, using a 40x/water objective and a 490 nm excitation filter for green signal [24 (link)]. Whole excised lungs after experimentation were mounted on glass sides without mounting media and imaged using a Zeiss LSM 710 Confocal Laser Scanning microscope at 40X/water objective. A red filter of excitation of 558 nm was used to identify Cell Trace labeled 4T1 cells attached to the lungs.
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Visualizing Aortic Glycocalyx and Endothelial Markers
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Other organizations : Northeastern University, Albert Einstein College of Medicine
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Variable analysis
independent variables
- Excitation filter wavelength (490 nm for green signal, 558 nm for red signal)
- GCX coverage and thickness
- Visualized localization of ZO-1, PECAM-1, E-selectin, and eNOS in en face aorta tissue samples
- Visualization and identification of Cell Trace labeled 4T1 cells attached to the lungs
- Magnification (63X/oil for aorta sections, 40X/water for en face aorta and whole excised lungs)
- Microscope (Zeiss LSM 710 Confocal Laser Scanning microscope)
- No positive or negative controls were explicitly mentioned in the provided information.
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