Quantifying F-actin and Morphology Changes in mDCs

IL-10-treated mDCs were stained with rhodamine phalloidin (Invitrogen, USA) and their F-actin structures and morphologies were examined on a confocal laser scanning microscope (Leica, Germany). Images were acquired and the F-actin contents were quantified by measuring the mean fluorescent intensities in each group. The lengths and numbers of cell membrane protrusions were also measured using Leica LAS Image Analysis software [28 (link)]. At least twenty cells in each group were selected for analysis.

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Xu X., Liu X., Long J., Hu Z., Zheng Q., Zhang C., Li L., Wang Y., Jia Y., Qiu W., Zhou J., Yao W, & Zeng Z. (2017). Interleukin-10 reorganizes the cytoskeleton of mature dendritic cells leading to their impaired biophysical properties and motilities. PLoS ONE, 12(2), e0172523.

Publication 2017

rhodamine phalloidin confocal laser scanning microscope LAS Image Analysis software

Cells Confocal laser scanning microscope F actin Il 10 Mdcs Membrane Rhodamine phalloidin

Corresponding Organization : Affiliated Hospital of Guizhou Medical University

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Variable analysis

independent variables

IL-10 treatment

dependent variables

F-actin structures and morphologies
F-actin contents (measured by mean fluorescent intensities)
Lengths and numbers of cell membrane protrusions

control variables

Not explicitly mentioned

controls

No positive or negative controls were explicitly mentioned in the provided information.

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