The significance of array results has been checked and validated by RT-PCR analysis for selected significantly regulated genes throughout the array study. The RT-PCR was performed with the same samples used for microarray according to QuantiTect SYBR Green Kit (Qiagen, Germany).37 (link),41 (link),44 (link),45 (link)
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Validating Microarray Results by RT-PCR
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Corresponding Organization :
Other organizations : German Cancer Research Center, Heidelberg University, Helwan University, Zagazig University, Arish University, Najran University, King Abdulaziz University, Delta University for Science and Technology, Suez Canal University, Prince Sattam Bin Abdulaziz University
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Variable analysis
independent variables
- Not explicitly mentioned
- The expression levels of significantly regulated genes, as measured by RT-PCR analysis
- The samples used for microarray analysis were the same as those used for the RT-PCR validation
- Not specified
- Not specified
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