Generating Hepatocyte-Specific Acly Knockout Mice

Generation of Acly^f/f mice on a C57Bl6/J background was previously described^{10 (link)}. To generate hepatocyte-specific Acly knockouts, Acly^f/f mice were crossed to albumin-Cre transgenic mice (B6.Cg-Tg(Alb-Cre)21Mgn/J, Jackson Laboratory)³⁷.

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Zhao S., Jang C., Liu J., Uehara K., Gilbert M., Izzo L., Zeng X., Trefely S., Fernandez S., Carrer A., Miller K.D., Schug Z.T., Snyder N.W., Gade T.P., Titchenell P.M., Rabinowitz J.D, & Wellen K.E. (2020). Dietary fructose feeds hepatic lipogenesis via microbiota-derived acetate. Nature, 579(7800), 586-591.

Publication 2020

B6.Cg-Tg(Alb-Cre)21Mgn/J

Albumin Hepatocyte Mice Transgenic mice

Corresponding Organization :

Other organizations : University of Pennsylvania, Princeton University, The Wistar Institute, Temple University

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Variable analysis

independent variables

Generation of Acly^(f/f) mice on a C57Bl6/J background
Crossing Acly^(f/f) mice to albumin-Cre transgenic mice (B6.Cg-Tg(Alb-Cre)21Mgn/J, Jackson Laboratory)

dependent variables

Hepatocyte-specific Acly knockouts

control variables

C57Bl6/J background
Acly^(f/f) mice

controls

No explicit positive or negative controls mentioned.

Annotations

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