Histological analysis was performed as described previously (27 (link)). At 4 weeks after HCHFD treatment and MCP interventions, all rats were sacrificed following anesthesia with Fentanyl (0.4 mg/kg; Sigma-Aldrich; Merck KGaA) and carotid artery vascular endothelial cells (CAVECs) were collected. CAVECs were fixed in 4% phosphate-buffered saline-buffered formalin for >24 h, followed by processing for conventional paraffin embedding. Sections (5-um thickness) were mounted on glass slides, dewaxed, rehydrated with distilled water and stained with hematoxylin and eosin. The CAVECs were examined using a light microscope to identify pathological changes.
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Histological Analysis of Carotid Artery Endothelial Cells
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Anesthesia
Carotid artery
Eosin
Fentanyl
Formalin
Light microscope
Phosphate
Rats
Saline
Vascular endothelial cells
Corresponding Organization :
Other organizations : Southern Medical University Shenzhen Hospital, Second People's Hospital of Yunnan Province, Peking University Shenzhen Hospital, Shandong First Medical University, University of Hong Kong, Peking University
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Variable analysis
independent variables
- HCHFD treatment
- MCP interventions
- Pathological changes in carotid artery vascular endothelial cells (CAVECs)
- Anesthesia with Fentanyl (0.4 mg/kg; Sigma-Aldrich; Merck KGaA)
- Fixation of CAVECs in 4% phosphate-buffered saline-buffered formalin for >24 h
- Conventional paraffin embedding of CAVECs
- Staining of CAVEC sections (5-um thickness) with hematoxylin and eosin
- Positive control: Not mentioned
- Negative control: Not mentioned
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