Once the ower buds had been disinfected, the next step was the dissection of the ower whorls: staminodes, petals, stamens and carpels in order to evaluate their morphogenic potential. The induction and expression of primary somatic embryogenesis was carried out by a modi cation of the protocol of Guiltinan et. al 2003, substituting thidiazuron (TDZ) for 6-benzylaminopurine (BAP) at a concentration of 1.4 µM in both the primary callus growth medium (PCG) and secondary callus (SCG), in order to evaluate this regulator growth in induction of embryogenesis. Caulogenesis was evaluated four weeks after inoculum. Rhizogenesis and primary somatic embryogenesis were evaluated at 14 weeks in the expression medium (ED). Secondary embryogenesis was induced and expressed according to Guiltinan et. al 2003 without modi cations from ve-week-old mature cotyledons in expression medium. Primary embryogenesis was evaluated in parallel using TDZ at a concentration of 22.7 nM according to Guiltinan et. al 2003 in the genotypes GPS3, GPS5 and GPS8 corresponding to T. cacao and in the genotypes GPS7, GPS9, GPS10, GPS11, GPS13, GPS15 and GPS16 of T. bicolor.