Immunoblotting Analysis of Cellular Signaling
Corresponding Organization : Queen's University
Other organizations : Kingston Health Sciences Centre
Variable analysis
- No independent variables are explicitly mentioned in the provided information.
- Levels of ezrin
- Levels of radixin
- Levels of moesin
- Levels of phospho-ezrin-radixin-moesin
- Levels of phospho-Serine473 Akt
- Levels of total Akt
- Levels of phospho-Serine136 Bad
- Levels of total Bad
- Levels of phospho-Serine536 p65 NFκB
- Levels of total p65 NFκB
- Levels of survivin
- Levels of γ-tubulin
- SDS-PAGE was performed as described previously (11)
- Proteins were separated by SDS-PAGE, transferred to 0.45 μm polyvinylidene difluoride membranes, blocked with 5% nonfat dry milk in 1× TBS/0.1% Tween-20
- No positive or negative controls are explicitly mentioned in the provided information.
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