Mouse Tissue RNA Extraction and Labeling

RNA isolation, amplification and labelling procedures were carried out essentially as described elsewhere [14 (link)]. Quality of tRNA was checked with a Bioanalyzer assay (RNA 6000 Pico Kit, Agilent Technologies, Amstelveen, The Netherlands). RNA integrity numbers of tRNA of mouse CH ranged from 4.9 to 6.9, of the mouse RPE ranged from 4.9 to 7.2 and of mouse PR ranged from 5.5 to 7.6. In our microarray study we used a common reference design. The common reference was prepared from mouse RPE/choroid that was isolated, amplified using the same methodology as our experimental samples, and labelled with Cy3 (Cy3 mono-reactive dye pack, GE Healthcare UK, Little Chalfont, Buckinghamshire, UK).
See Janssen et al. [14 (link)] for a more detailed description of the laser dissection procedures, RNA processing and microarray procedures.