All studies on fish were conducted in strict accordance with the institutional guidelines for animal research and approved by the Italian Ministry of Health; Department of Public Health, Animal Health, Nutrition and Food Safety in accordance to the law on animal experimentation (article 7; D.L. 116/92; protocol number: 00001/11/IGB; approval date June 6, 2011). Tricaine methanesulfonate (MS-222; Sigma Aldrich) was used for euthanasia (200-300mg/L) and anesthesia (0,05%) [41 (link)]. Furthermore, all animal treatments were reviewed and approved in advance by the Ethics Committee of the Institute of Genetics and Biophysics, IGB Animal House, (Naples, Italy).
Samples of the Cab strain of wild-type medaka fish were kept and staged as described previously [42 (link)]. Staging of retinal development in medaka fish was determined in accordance to Kitambi et al. [43 (link)].
Samples of the Cab strain of wild-type medaka fish were kept and staged as described previously [42 (link)]. Staging of retinal development in medaka fish was determined in accordance to Kitambi et al. [43 (link)].
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