Brain homogenates from four different CWD PrP allotypes were obtained from orally-infected white-tailed deer (O. virginianus) and homogenized in phosphate-buffered saline solution as described previously (15 (link)). Brains from transgenic mice were homogenized to 10% (w/v) in sterile water using ceramic beads and a tissue homogenizer (Omni, Kennesaw, GA). All aliquots were stored at −80 °C until needed. Mice were inoculated intracerebrally with 30 μl of 10% (w/v) brain homogenate unless otherwise denoted (24 (link)).
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