Termite specimens were homogenized in the pools (Table 1 ) using Tissue Lyser 2 (Qiagen, Hilden, Germany) for 15 min with frequency 25 s−1. High-throughput sequencing and the data-processing pipeline were the same as described previously [15 (link)].
RNA was extracted using TRI Reagent LS (Sigma, St. Louis, MA, USA) according to the manufacturer’s manual. After the extraction, host rRNA was depleted using an NEBNext Globin and rRNA Depletion Kit (NEB, E7750S, Ipswich, MA, USA) according to the manufacturer’s instructions. The obtained RNA was used for library preparation without polyA-enrichment using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770, Ipswich, MA, USA) according to the manufacturer’s instructions. Final libraries were sequenced (single-end, 250-nt reads) on a HiSeq1500 (Illumina, San Diego, CA, USA), and raw reads were deposited under the BioProject accession number PRJNA817887.
RNA was extracted using TRI Reagent LS (Sigma, St. Louis, MA, USA) according to the manufacturer’s manual. After the extraction, host rRNA was depleted using an NEBNext Globin and rRNA Depletion Kit (NEB, E7750S, Ipswich, MA, USA) according to the manufacturer’s instructions. The obtained RNA was used for library preparation without polyA-enrichment using a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, E7770, Ipswich, MA, USA) according to the manufacturer’s instructions. Final libraries were sequenced (single-end, 250-nt reads) on a HiSeq1500 (Illumina, San Diego, CA, USA), and raw reads were deposited under the BioProject accession number PRJNA817887.
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