Normal human fetal colonic mucosa cells (FHC) and CRC cells (SW620 and SW480) were bought from the American Type Culture Collection (ATCC, Manassas, VA, USA). 5-FU-resistant CRC cells (SW620/5-FU and SW480/5-FU) were generated by treating SW620 and SW480 cells with increasing doses of 5-FU (Solarbio, Beijing, China), as previously described.23 (link) All these cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM; HyClone, South Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; HyClone) and 1% penicillin-streptomycin (HyClone) at an atmosphere of 5% CO2 and 37°C. To maintain the resistance of SW620/5-FU and SW480/5-FU cells, 15.0 μg/mL 5-FU (Solarbio) was additionally added into the medium.
Protocol detail
Establishing 5-FU Resistant CRC Cell Lines
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization : First Affiliated Hospital of Zhengzhou University
Other organizations : Shangqiu First People's Hospital
Top 5 similar protocols
Variable analysis
independent variables
- Increasing doses of 5-FU (Solarbio, Beijing, China)
- Generation of 5-FU-resistant CRC cells (SW620/5-FU and SW480/5-FU)
- Normal human fetal colonic mucosa cells (FHC)
- CRC cells (SW620 and SW480)
- Dulbecco's modified Eagle's medium (DMEM; HyClone, South Logan, UT, USA) supplemented with 10% fetal bovine serum (FBS; HyClone) and 1% penicillin-streptomycin (HyClone)
- Atmosphere of 5% CO2 and 37°C
- SW620 and SW480 cells
- Normal human fetal colonic mucosa cells (FHC)
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!