Three‐ to four‐week‐old male Kunming mice were purchased from Dashuo Animal Center (Chengdu, China) and housed under specific pathogen‐free conditions. The animals were maintained according to specific protocols, and all euthanasia procedures were approved by the Guidelines for the Care and Use of Laboratory Animals and the Ethics Committee of Sichuan Agricultural University (Ya'an, China). The mice received either a normal diet or a high‐fat diet for 8 weeks. During the experiment, food and water were supplied ad libitum, except during 8‐h food deprivation periods before blood draws.