Quantification of Serum Short-Chain Fatty Acids

Difructose anhydride III was donated by Nippon Beet Sugar Manufacturing Co. Ltd., Obihiro, Japan. We prepared a mixture of short fatty acids-acetic acid, propionic acid, iso butyric acid, butyric acid, 2-methylbutanoic acid, iso valeric acid, valeric acid, caproic acid, 2-methylhexanelic acid, and enanthic acid (Sigma Aldrich, Tokyo, Japan ) to measure SCFAs in the serum samples using gas chromatography-mass spectrometry (GC/MS) according to previous reports on the concentrations of SCFAs in serum samples or rumen fluids of cattle [9 (link), 23 (link), 24 (link)].

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Priyo TW J.r., Uno S., Kokushi E., Toda K., Hasunuma H., Matsumoto D., Yamato O., Ohtani M., Widodo O.S., Pambudi D., Taniguchi M, & Takagi M. (2023). Measurement of serum short-chain fatty acid concentrations in cattle after oral administration of difructose anhydride III. Veterinary World, 16(7), 1505-1511.

Publication 2023

acetic acid propionic acid isobutyric acid butyric acid 2-methylbutanoic acid isovaleric acid valeric acid caproic acid enanthic acid

2 methylbutanoic acid Acetic acids Acid Beet sugar Butyric acid Caproic acid Cattle Difructose anhydride iii Gas chromatography mass spectrometry Isobutyric acid Isovaleric acid Propionic acid Rumen Serum Valeric acid

Corresponding Organization :

Other organizations : Universitas Gadjah Mada, Yamaguchi University, Kagoshima University, National Livestock Breeding Center, Airlangga University, Sebelas Maret University

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Variable analysis

independent variables

Difructose anhydride III
Short fatty acids-acetic acid, propionic acid, isobutyric acid, butyric acid, 2-methylbutanoic acid, isovaleric acid, valeric acid, caproic acid, 2-methylhexanelic acid, and enanthic acid

dependent variables

Concentrations of short-chain fatty acids (SCFAs) in serum samples

control variables

Positive and negative controls not explicitly mentioned

controls

Positive controls not specified
Negative controls not specified

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