Antibody Generation for VEGFA and AGO1

Antibodies specific to the readthrough region of VEGFA (against the peptide AGLEEGASLRVSGTR) and AGO1 (against the peptide RQNAVTSLDRRKLSKP) were generated as described in previous studies (Eswarappa et al, 2014 (link); Singh et al, 2019 (link)). Anti-Ago1 antibody (Novus Biologicals, NB100-2817), Anti-GFP antibody (BioLegend, 902602), anti-puromycin antibody (PMY-2A4, Developmental Studies Hybridoma Bank), anti-p53 antibody (AHO0152, Thermo Fisher), anti-FLAG antibody (Sigma, F1804), anti-Spectrin alpha-1 antibody (Invitrogen, ARC1650), anti-HA antibody (Sigma, 11867423001), anti-Actin antibody (Sigma, A3854) and horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher/Jackson Immunoresearch labs) were used as per the manufacturer’s instructions.

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Manjunath L.E., Singh A., Devi Kumar S., Vasu K., Kar D., Sellamuthu K, & Eswarappa S.M. (2024). Transcript-specific induction of stop codon readthrough using a CRISPR-dCas13 system. EMBO Reports, 25(4), 2118-2143.

Publication 2024

Anti-GFP antibody anti-FLAG antibody anti-HA antibody anti-Actin antibody

Corresponding Organization : Indian Institute of Science Bangalore

Other organizations : Northwestern University, The University of Texas Medical Branch at Galveston

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Variable analysis

independent variables

Antibodies specific to the readthrough region of VEGFA (against the peptide AGLEEGASLRVSGTR)
Antibodies specific to AGO1 (against the peptide RQNAVTSLDRRKLSKP)

dependent variables

Not explicitly mentioned

control variables

Anti-Ago1 antibody (Novus Biologicals, NB100-2817)
Anti-GFP antibody (BioLegend, 902602)
Anti-puromycin antibody (PMY-2A4, Developmental Studies Hybridoma Bank)
Anti-p53 antibody (AHO0152, Thermo Fisher)
Anti-FLAG antibody (Sigma, F1804)
Anti-Spectrin alpha-1 antibody (Invitrogen, ARC1650)
Anti-HA antibody (Sigma, 11867423001)
Anti-Actin antibody (Sigma, A3854)
Horseradish peroxidase-conjugated secondary antibodies (Thermo Fisher/Jackson Immunoresearch labs)

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

Annotations

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