Lumefantrine was mixed with 1 mL Miglyol® 812 N and sonicated for 2 min at 50% amplitude. In a different Eppendorf tube, LF and Miglyol® 812 N were mixed first followed by sonication at 50% amplitude for 2 min. After LF was dissolved, AR was added to the solution and the sample was sonicated again for 2 min. Nuclear magnetic resonance (NMR) samples of the pure compounds (LF, AR, Miglyol® 812 N) as well as of the precipitates, both from LF alone in Miglyol® 812 N, and from a mixture of LF and AR in Miglyol® 812 N, were prepared in deuterated chloroform (CDCl3) and their 1H spectra were recorded on a Bruker Avance 400 MHz spectrometer and analyzed using MestReNova software.
Protocol detail
NMR Analysis of Lumefantrine-Artesunate Mixture
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Corresponding Organization : Kamuzu Central Hospital
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Variable analysis
independent variables
- Sonication time (2 min)
- Sonication amplitude (50%)
- Dissolution of Lumefantrine (LF)
- Precipitation of LF and Artesunate (AR) in Miglyol® 812 N
- Volume of Miglyol® 812 N (1 mL)
- Deuterated chloroform (CDCl3) for NMR sample preparation
- Positive control: NMR spectra of pure compounds (LF, AR, Miglyol® 812 N)
- Negative control: NMR spectra of precipitates from LF alone in Miglyol® 812 N, and from a mixture of LF and AR in Miglyol® 812 N
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