Lanosterol Biosynthesis in Leishmania

Lanosterol (>=93% pure) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) were obtained from Sigma-Aldrich (St. Louis, MO). 5α-Cholesta-8,24-dien-3β-ol (zymosterol), 14-demethyl-14-dehydroLanosterol (FF-MAS), 4,4-dimethylcholesta-8,24-dien-3β-ol (T-MAS), and cholesterol-d7 were purchased from Avanti Polar Lipids Inc (Alabaster, AL). 4α,14α-Dimethylzymosterol (4,14-DMZ) was isolated from the sterol extract of L. tarentolae [16 (link)]. Dilaurylphosphatidylcholine (DLPC), dimyristoylphosphatidylcholine (DMPC), and dimyristoylphosphatidylglycerol (DMPG) were a gift from Dr. Philip Gao at the Protein Production Group, University of Kansas. Emulgen 911 was purchased from Desert Biologicals (Phoenix, AZ). Reagents used in Leishmania work were purchased from Thermo Fisher Scientific (Waltham, MA) or VWR (Radnor, PA) unless otherwise specified.

Free full text: Click here

Jin Y., Basu S., Feng M., Ning Y., Munasinghe I., Joachim A.M., Li J., Madden R., Burks H., Gao P., Perera C., Werbovetz K.A., Zhang K, & Wang M.Z. (2023). CYP5122A1 encodes an essential sterol C4-methyl oxidase in Leishmania donovani and determines the antileishmanial activity of antifungal azoles. Research Square.

Publication Preprint 2023

Lanosterol reduced nicotinamide adenine dinucleotide phosphate (NADPH) cholesterol-d7

Alabaster Biologicals Cholesterol Dilaurylphosphatidylcholine Dimyristoylphosphatidylcholine Dimyristoylphosphatidylglycerol Emulgen 911 Ff mas Lanosterol Leishmania Lipids Nicotinamide adenine dinucleotide phosphate Protein Sterol T mas Zymosterol

Corresponding Organization : University of Kansas

Other organizations : Texas Tech University, The Ohio State University

Top 5 similar protocols

Variable analysis

independent variables

Lanosterol (>=93% pure)
Reduced nicotinamide adenine dinucleotide phosphate (NADPH)
5α-Cholesta-8,24-dien-3β-ol (zymosterol)
14-demethyl-14-dehydrolanosterol (FF-MAS)
4,4-dimethylcholesta-8,24-dien-3β-ol (T-MAS)
4α,14α-Dimethylzymosterol (4,14-DMZ)
Dilaurylphosphatidylcholine (DLPC)
Dimyristoylphosphatidylcholine (DMPC)
Dimyristoylphosphatidylglycerol (DMPG)
Emulgen 911

dependent variables

Not explicitly mentioned

control variables

Cholesterol-d7

positive controls

Not specified

negative controls

Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!