Longitudinal Monitoring of CAR T Cells

CAR T cells were longitudinally monitored in peripheral blood (PB) through multiparameter flow cytometry (MFC) using CD19 CAR detection reagent, as previously described,¹¹ (link) or CD19 CAR FMC63 Idiotype (REA1297) (both from Miltenyi Biotec). CAR T-cell differentiation in infusion product leftovers was assessed by MFC as previously described.¹¹ (link) Antibodies and gating strategies are detailed in supplemental Material. Data were acquired on BD FACSCanto II (BD Biosciences), MACSQuant Analyzer MQ10 or MQ16 (Miltenyi Biotec) and analyzed using FlowJo-v10 and MACSQuantify softwares.

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Carniti C., Caldarelli N.M., Agnelli L., Torelli T., Ljevar S., Jonnalagadda S., Zanirato G., Fardella E., Stella F., Lorenzini D., Brich S., Arienti F., Dodero A., Chiappella A., Magni M, & Corradini P. (2024). Monocytes in leukapheresis products affect the outcome of CD19–targeted CAR T-cell therapy in patients with lymphoma. Blood Advances, 8(8), 1968-1980.

Publication 2024

CD19 CAR detection reagent BD FACSCanto II MACSQuant Analyzer MQ10

Corresponding Organization :

Other organizations : Fondazione IRCCS Istituto Nazionale dei Tumori, University of Milan

Top 5 similar protocols

Variable analysis

independent variables

CAR T cells

dependent variables

CAR T-cell differentiation in infusion product leftovers
CAR T cells in peripheral blood (PB)

control variables

CD19 CAR detection reagent
CD19 CAR FMC63 Idiotype (REA1297) from Miltenyi Biotec
Antibodies and gating strategies detailed in supplemental Material
Flow cytometry instruments (BD FACSCanto II, MACSQuant Analyzer MQ10 or MQ16)
Flow cytometry analysis software (FlowJo-v10 and MACSQuantify)

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

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