Plasmid and Genomic DNA Isolation and Analysis

Plasmid DNA from bacterial cells was isolated using E.Z.N.A. Plasmid Mini Kit I (Omega Bio-tek). Genomic DNA from mammalian cells was isolated using E.Z.N.A. Tissue DNA Kit (Omega Bio-tek). Amplification of the DNA fragments for cloning was performed using Pfu-Ultra polymerase (Agilent). PCR analysis of the genomic DNA was performed using Q5 (New England Biolabs) and GoTag (Promega) master mixes. The ClustalW program (https://www.genome.jp/tools-bin/clustalw) was used for sequence alignments. Golden Gate TALEN kit was used to assemble the DNA binding domains of TAL^{28 (link)}. General genetic engineering experiments were performed as described in Molecular Cloning Manual⁵¹ . 3D structures of the proteins were analyzed using Swiss-PdbViewer^{52 (link)}.