Expression of GFP and GDH Constructs

Plasmids pSMCAF015 and pSMCAF016 used for the expression of GFP and SpyCatcher-GFP from E. coli were assembled from existing constructs (pBAD-RFP and pBAD-SpyCatcher-RFP^{29 (link)}, respectively) by substituting the mRFP sequence with the GFP sequence (below). Similarly, plasmids pSMCAF032 and pSMCAF029 used for the expression of GDH and SpyCatcher-GSH from E. coli were assembled by introducing the GDH sequence in the same position. These plasmids were transformed into chemically competent BL21(DE3) cells (New England Biolabs – C2527H); single transformants were selected using ampicillin resistance.